Shotgun Sequencing (also known as Sequencing, Shotgun, Sequencings, Shotgun): A laboratory technique for determining the DNA sequence of an organism's genome. In this approach, DNA of an organism is randomly broken into small DNA fragments which are then sequenced individually. Computational methods are then utilized t

NIH MeSH · D000098625Analytical, Diagnostic and Therapeutic Techniques and Equipment

Shotgun Sequencing

Also known asSequencing, Shotgun · Sequencings, Shotgun · Shotgun Sequencings · Whole Genome Shotgun · Whole Genome Shotgun Sequencing

Definition

A laboratory technique for determining the DNA sequence of an organism's genome. In this approach, DNA of an organism is randomly broken into small DNA fragments which are then sequenced individually. Computational methods are then utilized to reassemble these sequenced fragments accurately, reconstructing the complete genome sequence.

MeSH classification

  • E05.393.940

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Common questions about Shotgun Sequencing

What does Shotgun Sequencing mean in medicine?
A laboratory technique for determining the DNA sequence of an organism's genome. In this approach, DNA of an organism is randomly broken into small DNA fragments which are then sequenced individually. Computational methods are then utilized to reassemble these sequenced fragments accurately, reconstructing the complete genome sequence. This definition is taken from the National Library of Medicine's MeSH (Medical Subject Headings) 2026 release, which is the standard vocabulary used to index PubMed and most medical literature.
Is Shotgun Sequencing known by any other names?
Yes. Shotgun Sequencing is also referred to as Sequencing, Shotgun, Sequencings, Shotgun, Shotgun Sequencings, Whole Genome Shotgun in different clinical, research and patient-facing contexts. The MeSH descriptor groups all of these synonyms under a single canonical concept so research and records stay consistent.
Where does Shotgun Sequencing sit in the medical classification?
Shotgun Sequencing falls under the broader medical category "Analytical, Diagnostic and Therapeutic Techniques and Equipment" in the MeSH hierarchy (tree numbers: E05.393.940). Browsing the related concepts on this page takes you to neighbouring topics in the same branch of medicine.
Where can I get a plain-language explanation of Shotgun Sequencing?
For a plain-language explanation of Shotgun Sequencing - including symptoms, treatments and what it means for an Indian patient - ask GoDavaii's Health AI. It works in 22+ Indian languages, is free and needs no signup. Tap "Ask GoDavaii AI about Shotgun Sequencing" above.

Source: NIH MeSH 2026 (D000098625) — National Library of Medicine, public domain. View official MeSH record ↗